بررسی اثر محافظتی میریستین بر آسیب بیوشیمیایی کبد ناشی از اندوسولفان در شرایط درون تنی

Background and purpose: Endosulfan is an organochlorine insecticide that is absorbed orally, subcutaneously and by inhalation in humans and animals and disturbs the reduction process, changes the activity of antioxidant enzymes and increases the lipid peroxidation in many organs. The aim of this study was to evaluate the protective effect of myricetin against endosulfan induced liver damage. Materials and methods: In an experimental study 42 male Wister rats were randomly divided into 7 groups (n=6 per group). Group I served as the control and the experimental groups were as follows: group II- Ѵ received endosulfan 10 mg/kg and myricetin 5, 10, 15, and 20 mg/kg/ip for 30 days, respectively. Group IѴwas treated only by 10 mg/kg endosulfan and group IIѴ as positive control received 10 mg/kg cyclophosphamide (ip, 30 days). Twenty four hours after the last injection, all rats were anesthetized using ethyl ether. Alanine aminotransferase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), and superoxide dismutase (SOD) levels were measured in plasma. Liver glutathione (GSH) levels were also determined. Results: Administration of endosulfan caused elevation in ALT, AST, ALP and SOD levels, but the activity of GSH decreased in liver tissue. Receiving myricetin along with endosulfan significantly increased body antioxidant activity and normalized the liver enzyme levels (P˂ 0.05). Conclusion: Our results indicated that myricetin with antioxidant effect could protect cells against harmful free radicals. Therefore, further clinical trials are suggested.